Surface Properties of Top- and Bottom-Fermenting Yeast

The surface physico-chemical properties (hydrophobicity, electrophoretic mobility, chemical composition) of a large set of top- and bottom-fermenting brewing yeasts, harvested in the exponential and stationary growth phases, have been investigated. Bottom- and top-fermenting strains showed different surface properties. Top strains were generally more hydrophobic than bottom strains, due to higher surface protein concentrations. Bottom strains possessed higher surface phosphate concentrations. The different profiles of electrophoretic mobility versus pH for top and bottom strains could be explained by modelling the surface charge according to the surface chemical composition as given by X-ray photoelectron spectroscopy. For bottom strains, the electrical properties were mainly controlled by phosphate, resulting in a low isoelectric point (pH 2 or below) and an electrophoretic mobility that did not become much more negative above pH 4. For the top strains, they were mainly determined by the balance of protonated amino- and carboxylate groups in proteins, which gave a high isoelectric point (pH 4) and an electrophoretic mobility changing greatly with pH in the range of 2 to 7. No difference in surface properties was found between flocculating and non-flocculating strains, or between cells from the exponential and stationary growth phases, even for strains where flocculation occurred during the transition from one growth phase to the other. © 1997 John Wiley & Sons, Ltd.     

Mesure de largeurs de trait en éclairage partiellement cohérent par modélisation du transfert et utilisation de techniques de moindres carrés

A method of transfer modelling in partially coherent microscopy is presented. By determining the minimal error between simulated and experimental data by means of a lms technique, it is then possible to retrieve, with accuracy, the linewidth on wafers.     

Reaction of α, ω-dicarboxy polyamides in the melt: synthesis of a model

Summary The synthesis of N-dodecanoyl-11-aminoundecanoic acid as a model for carboxylic polyamides 11 is described. The solubilization reaction of these polyamides with trifluoroacetic anhydride is studied by ¹H and ¹³C NMR spectroscopy. The resulting soluble trifluoroacetylated compounds are stable enough to allow an easy GPC study in CH₂Cl₂ on routine instument and columns. The participation of the residual dodecanoic acid in the polycondensation equilibrium of carboxylic polyamides has been established.     

The relationship between fatty acid peroxidation and α-tocopherol consumption in isolated normal and transformed hepatocytes

The response of normal and transformed rat hepatocytes to oxidative stress was investigated. Isolated normal rat hepatocytes and differentiated hepatoma cells (the Fao cell line was derived from the Reuber H 35 rat hepatoma) in suspension were incubated with the ADP/Fe³⁺ chelate for 30 min at 37°C. Membrane lipid oxidation was assessed by measuring (i) free malondialdehyde (MDA) production by a high-performance liquid chromatography (HPLC) procedure, (ii) membrane fatty acid disappearance as judged by capillary gas chromatography, and (iii) α-tocopherol oxidation as determined by HPLC and electrochemical detection. The addition of iron led to increased MDA production in normal as well as in transformed cells, and to simultaneous consumption of polyunsaturated fatty acids (PUFA) and α-tocopherol. In addition, in Fao cells more α-tocopherol was consumed during lipid peroxidation while less PUFA was oxidized. Lipid peroxidation was lower in tumoral hepatocytes than in normal cells. This could be due to a difference in membrane lipid composition because of a lower PUFA content and a higher α-tocopherol level in Fao cells. During oxidation, Fao cells produced 1.5 to 2 times less MDA than normal cells, while in the tumoral cells the amount of oxidized PUFA having 3 or more double bonds was 7 to 8 times lower. Therefore, measuring MDA alone as an index of lipid peroxidation did not allow for proper comparison of the membrane lipid oxidizability of transformed cellsvs. the membrane lipid oxidizability of normal cells.     

Three-Dimensional Numerical Modeling of Mixing at River Confluences

Lateral mixing of a pollutant is considered as a slow process that is usually complete within 100–300 river widths. Recent studies on flow dynamics at river confluences revealed that lateral mixing can be markedly enhanced when the tributary channel is shallower than the main channel. This study uses a three-dimensional model to examine mixing processes immediately downstream of confluences as well as further downstream in the mainstream. Simulations are presented for a concordant and discordant laboratory junction and a field confluence for a low and a high flow condition. The decrease in standard deviation at a cross section of a tracer over a distance of 5 channel widths is 30% for discordant beds but only 10% for concordant beds in the laboratory simulation. At the natural site, bed discordance is more important at the low flow than at the high flow with corresponding decreases in the standard deviation of 31 and 18% over 3.5 channel widths. Mixing is completed after a distance of 25 and 37 channel widths for the low and high flow conditions, respectively. Further downstream, mixing is mainly affected by planform curvature of the channel.     

Soy protein isolate nanocomposite film enriched with eugenol,an antimicrobial agent: Interactions and properties

Nanocomposites films were designed from soy protein isolates (SPI), clays (Na⁺‐MMT), and eugenol an antimicrobial agent. Interactions between Na⁺‐MMT and eugenol were evidenced by a shift of the d‐spacing by X‐ray diffraction analysis. The addition of Na⁺‐MMT (5 and 7.5% w/w) in SPI solution increased its shear thinning behavior and its consistency. Accordingly, a good exfoliation of clays in SPI films was observed. The glass transition temperature of SPI films was impacted by the clays addition but not the water vapor permeability. In contrast, the addition of eugenol in SPI solution did not affected the consistency but induced a decrease of the SPI film T_g and an increase of the water vapor permeability. The presence of eugenol counterbalanced the effect of clays on consistency of film‐forming solution. The clay intercalation process was facilitated and the water vapor permeability and active agent release were modified. The presence of clay did not affect the antibacterial effect of eugenol/SPI films. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018 , 135, 45941.     

Offset printing inks based on rapeseed oil and sunflower oil. Part II: Varnish and ink formulation

Two sets of variable oil length, alkyd resins modified by sunflower oil (SOA) and by rapeseed oil (ROA), were evaluated in offset formulations with mineral oils as diluent. The more suitable alkyds for this kind of application were determined. In a second experiment, hydrocarbon solvents were substituted by the fatty acid methyl esters derived from rapeseed oil or sunflower oil to produce ecologically friendly offset printing inks. Finally, the ROA and the SOA were associated with the methyl esters derived from the same vegetable oil. New properties of the varnishes composed of a vegetable diluent were evaluated. The quickset formulations with the methyl esters do not need important modifications, as opposed to the heatset formulations.     

Pine forest investigation using high resolution P-band Pol-InSAR data

A Pol-InSAR dataset acquired at P-band over a maritime pine forest (Nezer forest of Landes, France) has been investigated and compared with tree structural ground measurements in order to identify scatterers associated with elementary scattering mechanisms in the canopy layer. Since this managed forest is constituted by different height stands, it allows to investigate mechanism change in the vertical direction during the forest growth. It has been observed that VV channel was the most elevated, which can be accounted for a maximum attenuation in the vertical direction and partially for the presence of sub-vertical branches in the top canopy layer, and that this channel was the most sensitive to the tree density in reason of an important ground reflectivity. The most pure double-bounce scattering mechanism component is received in HH-VV channel where the corresponding phase center reaches the ground level for large Diameter at Breast Height. It is also supposed that HH channel, which receives double bounce backscatter, was affected by the sub-horizontal primary branch development, which induces an elevation of the phase center over oldest trees while tree density diminution enhances backscatter from the ground level. Finally, the crosspolar channel appeared the less perturbed by the tree density than VV channel since it receives predominantly the canopy contribution.     

Self-ordered particle trains in inertial microchannel flows

Controlling the transport of particles in flowing suspensions at microscale is of interest in numerous contexts such as the development of miniaturized and point-of-care analytical devices (in bioengineering, for foodborne illnesses detection, etc.) and polymer engineering. In square microchannels, neutrally buoyant spherical particles are known to migrate across the flow streamlines and concentrate at specific equilibrium positions located at the channel centerline at low flow inertia and near the four walls along their symmetry planes at moderate Reynolds numbers. Under specific flow and geometrical conditions, the spherical particles are also found to line up in the flow direction and form evenly spaced trains. In order to statistically explore the dynamics of train formation and their dependence on the physical parameters of the suspension flow (particle-to-channel size ratio, Reynolds number and solid volume fraction), experiments have been conducted based on in situ visualizations of the flowing particles by optical microscopy. The trains form only once particles have reached their equilibrium positions (following lateral migration). The percentage of particles in trains and the interparticle distance in a train have been extracted and analyzed. The percentage of particles organized in trains increases with the particle Reynolds number up to a threshold value which depends on the concentration and then decreases for higher values. The average distance between the surfaces of consecutive particles in a train decreases as the particle Reynolds number increases and is independent of the particles size and concentration, if the concentration remains below a threshold value related to the degree of confinement of the suspension flow.